Reuse of Immobilized <i>Komagataella phaffii</i> Cells for the Elimination of <scp>d</scp>-Glucose in Syrups of Bioactive Carbohydrates

During the synthesis of prebiotic carbohydrates such as fructooligosaccharides (FOS), galactooligosaccharides (GOS), or isomaltooligosaccharides (IMOS), d-glucose is released a side-product transglycosylation process. It desirable to remove glucose from these sugar mixtures due its caloric contribution and effect on caries diabetes. In this work, we have investigated use immobilized Komagataella phaffii (formerly Pichia pastoris) for elimination d-fructose in several syrups. K. cells were calcium alginate beads facilitate separation yeast reaction medium reuse biocatalyst. The yeasts successfully reutilized at least 20 cycles (of 195 min) (62.3 g/L) (5.5 FOS syrup, without affecting concentration oligosaccharides. Excellent selectivity was also found (57.2 IMOS methodology versatile easy scale-up, demonstrated removal (97.5 (142 purification heteroglucooligosaccharides synthesized by Metschnikowia reukaufii α-glucosidase. addition, (50 selectively removed presence d-galactose 150 min applied GOS purification.